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pc3 luciferase pc3 luc2  (ATCC)


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    ATCC pc3 luciferase pc3 luc2
    Fig. 1. Piezo1 expression is enhanced by wall shear stress (A) The prostate tumor microenvironment was immunohistochemically stained to evaluate Piezo1 expression. Scale bars represent 250 μm and 25 μm, respectively. (B) <t>PC3</t> human prostate cancer cells were plated in the microfluidics channel and applied shear stress at 0.05 dyne cm−2 for 5 h. After wall shear stress (WSS), gene expression of Piezo1 was confirmed by qRT-PCR (n = 3, independent experiments, two-tailed t- test, *** p < 0.001). (C) Immunofluorescence staining analysis was performed to evaluate Piezo1 expression (red). Cell nuclei were stained with DAPI (blue). Scale bars represent 10 μm. (D) Protein expression of Piezo1 was detected using immunoblotting at 0, 1, 5, and 12 h after WSS.
    Pc3 Luciferase Pc3 Luc2, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pc3+luciferase+pc3+luc2/PC-3-Luc2/pm36084759-45-1-13
    Average 92 stars, based on 7 article reviews
    pc3 luciferase pc3 luc2 - by Bioz Stars, 2026-10
    92/100 stars

    Images

    1) Product Images from "Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis."

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.

    Journal: Life sciences

    doi: 10.1016/j.lfs.2022.120936

    Fig. 1. Piezo1 expression is enhanced by wall shear stress (A) The prostate tumor microenvironment was immunohistochemically stained to evaluate Piezo1 expression. Scale bars represent 250 μm and 25 μm, respectively. (B) PC3 human prostate cancer cells were plated in the microfluidics channel and applied shear stress at 0.05 dyne cm−2 for 5 h. After wall shear stress (WSS), gene expression of Piezo1 was confirmed by qRT-PCR (n = 3, independent experiments, two-tailed t- test, *** p < 0.001). (C) Immunofluorescence staining analysis was performed to evaluate Piezo1 expression (red). Cell nuclei were stained with DAPI (blue). Scale bars represent 10 μm. (D) Protein expression of Piezo1 was detected using immunoblotting at 0, 1, 5, and 12 h after WSS.
    Figure Legend Snippet: Fig. 1. Piezo1 expression is enhanced by wall shear stress (A) The prostate tumor microenvironment was immunohistochemically stained to evaluate Piezo1 expression. Scale bars represent 250 μm and 25 μm, respectively. (B) PC3 human prostate cancer cells were plated in the microfluidics channel and applied shear stress at 0.05 dyne cm−2 for 5 h. After wall shear stress (WSS), gene expression of Piezo1 was confirmed by qRT-PCR (n = 3, independent experiments, two-tailed t- test, *** p < 0.001). (C) Immunofluorescence staining analysis was performed to evaluate Piezo1 expression (red). Cell nuclei were stained with DAPI (blue). Scale bars represent 10 μm. (D) Protein expression of Piezo1 was detected using immunoblotting at 0, 1, 5, and 12 h after WSS.

    Techniques Used: Expressing, Shear, Staining, Gene Expression, Quantitative RT-PCR, Two Tailed Test, Immunofluorescence, Western Blot

    Fig. 5. Piezo1 induces YAP activation through tyrosine 357 YAP and Src phosphorylation (A) and (B) Immunoblot analysis was measured after treatment with mock, a Piezo1 agonist (Yoda1, 1 μM), and an Src inhibitor (dasatinib, 100 nM) and exposure to static or WSS for 5 h. (C) and (D) Immunoblot analysis was measured after exposure to WSS for 5 h in PC3 cells transfected with siControl or siPiezo1. (B) and (D) Graphs showing quantification of the expression level of each phosphorylated protein compared with the expression of the non-phosphorylated isoform. (n = 3, Two Way ANOVA with Holm-Sidak multiple comparisons, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
    Figure Legend Snippet: Fig. 5. Piezo1 induces YAP activation through tyrosine 357 YAP and Src phosphorylation (A) and (B) Immunoblot analysis was measured after treatment with mock, a Piezo1 agonist (Yoda1, 1 μM), and an Src inhibitor (dasatinib, 100 nM) and exposure to static or WSS for 5 h. (C) and (D) Immunoblot analysis was measured after exposure to WSS for 5 h in PC3 cells transfected with siControl or siPiezo1. (B) and (D) Graphs showing quantification of the expression level of each phosphorylated protein compared with the expression of the non-phosphorylated isoform. (n = 3, Two Way ANOVA with Holm-Sidak multiple comparisons, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

    Techniques Used: Activation Assay, Phospho-proteomics, Western Blot, Transfection, Expressing

    Fig. 6. In vivo monitoring of orthotopic PC3 pros tate tumor growth and metastasis with or without shPiezo1. (A) Representative whole-body images of bioluminescence measurements for xenografted PC3 tumor growth and metastasis. Luciferase tag ged PC3-Luc/shCon and PC3-Luc/shPiezo1 cells were surgically implanted into the dorsal prostate of male nude mice. Ventral images of anesthetized representative sham, control (PC3-Luc/shCon), and shPiezo1 (PC3-Luc/shPiezo1) were taken weekly for 6 weeks to monitor spreading to other organs. All images were pseudo color set using the same intensity (sham = 4, shCon = 6, shPiezo1 = 6). (B) Histological slides include orthotopic tumors of PC3-luc/shCon and PC3-luc/shPiezo1. Representa tive images were derived from mouse prostate stained with hematoxylin and eosin (H&E). High magnified images of the top panel were presented as below. Scale bars = 25 μm and 100 μm, respectively.
    Figure Legend Snippet: Fig. 6. In vivo monitoring of orthotopic PC3 pros tate tumor growth and metastasis with or without shPiezo1. (A) Representative whole-body images of bioluminescence measurements for xenografted PC3 tumor growth and metastasis. Luciferase tag ged PC3-Luc/shCon and PC3-Luc/shPiezo1 cells were surgically implanted into the dorsal prostate of male nude mice. Ventral images of anesthetized representative sham, control (PC3-Luc/shCon), and shPiezo1 (PC3-Luc/shPiezo1) were taken weekly for 6 weeks to monitor spreading to other organs. All images were pseudo color set using the same intensity (sham = 4, shCon = 6, shPiezo1 = 6). (B) Histological slides include orthotopic tumors of PC3-luc/shCon and PC3-luc/shPiezo1. Representa tive images were derived from mouse prostate stained with hematoxylin and eosin (H&E). High magnified images of the top panel were presented as below. Scale bars = 25 μm and 100 μm, respectively.

    Techniques Used: In Vivo, Luciferase, Control, Derivative Assay, Staining

    Related Articles

    Expressing:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).

    Shear:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).

    Staining:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).

    Gene Expression:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).

    Quantitative RT-PCR:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).

    Two Tailed Test:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).

    Immunofluorescence:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).

    Western Blot:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).

    Activation Assay:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).

    Phospho-proteomics:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).

    Transfection:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).

    In Vivo:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).

    Luciferase:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).

    Control:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).

    Derivative Assay:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.
    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).. LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).LNCaP and 22Rv1 human prostate cancer cells were donated by Dr. Choung-Soo Kim (Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea).



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    pc3 luciferase pc3 luc2  (ATCC)
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    ATCC pc3 luciferase pc3 luc2
    Fig. 1. Piezo1 expression is enhanced by wall shear stress (A) The prostate tumor microenvironment was immunohistochemically stained to evaluate Piezo1 expression. Scale bars represent 250 μm and 25 μm, respectively. (B) <t>PC3</t> human prostate cancer cells were plated in the microfluidics channel and applied shear stress at 0.05 dyne cm−2 for 5 h. After wall shear stress (WSS), gene expression of Piezo1 was confirmed by qRT-PCR (n = 3, independent experiments, two-tailed t- test, *** p < 0.001). (C) Immunofluorescence staining analysis was performed to evaluate Piezo1 expression (red). Cell nuclei were stained with DAPI (blue). Scale bars represent 10 μm. (D) Protein expression of Piezo1 was detected using immunoblotting at 0, 1, 5, and 12 h after WSS.
    Pc3 Luciferase Pc3 Luc2, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pc3 luciferase pc3 luc2 human prostate cancer cells  (ATCC)
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    ATCC pc3 luciferase pc3 luc2 human prostate cancer cells
    Fig. 1. Piezo1 expression is enhanced by wall shear stress (A) The prostate tumor microenvironment was immunohistochemically stained to evaluate Piezo1 expression. Scale bars represent 250 μm and 25 μm, respectively. (B) <t>PC3</t> human prostate cancer cells were plated in the microfluidics channel and applied shear stress at 0.05 dyne cm−2 for 5 h. After wall shear stress (WSS), gene expression of Piezo1 was confirmed by qRT-PCR (n = 3, independent experiments, two-tailed t- test, *** p < 0.001). (C) Immunofluorescence staining analysis was performed to evaluate Piezo1 expression (red). Cell nuclei were stained with DAPI (blue). Scale bars represent 10 μm. (D) Protein expression of Piezo1 was detected using immunoblotting at 0, 1, 5, and 12 h after WSS.
    Pc3 Luciferase Pc3 Luc2 Human Prostate Cancer Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
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    92/100 stars
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    Image Search Results


    Fig. 1. Piezo1 expression is enhanced by wall shear stress (A) The prostate tumor microenvironment was immunohistochemically stained to evaluate Piezo1 expression. Scale bars represent 250 μm and 25 μm, respectively. (B) PC3 human prostate cancer cells were plated in the microfluidics channel and applied shear stress at 0.05 dyne cm−2 for 5 h. After wall shear stress (WSS), gene expression of Piezo1 was confirmed by qRT-PCR (n = 3, independent experiments, two-tailed t- test, *** p < 0.001). (C) Immunofluorescence staining analysis was performed to evaluate Piezo1 expression (red). Cell nuclei were stained with DAPI (blue). Scale bars represent 10 μm. (D) Protein expression of Piezo1 was detected using immunoblotting at 0, 1, 5, and 12 h after WSS.

    Journal: Life sciences

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.

    doi: 10.1016/j.lfs.2022.120936

    Figure Lengend Snippet: Fig. 1. Piezo1 expression is enhanced by wall shear stress (A) The prostate tumor microenvironment was immunohistochemically stained to evaluate Piezo1 expression. Scale bars represent 250 μm and 25 μm, respectively. (B) PC3 human prostate cancer cells were plated in the microfluidics channel and applied shear stress at 0.05 dyne cm−2 for 5 h. After wall shear stress (WSS), gene expression of Piezo1 was confirmed by qRT-PCR (n = 3, independent experiments, two-tailed t- test, *** p < 0.001). (C) Immunofluorescence staining analysis was performed to evaluate Piezo1 expression (red). Cell nuclei were stained with DAPI (blue). Scale bars represent 10 μm. (D) Protein expression of Piezo1 was detected using immunoblotting at 0, 1, 5, and 12 h after WSS.

    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

    Techniques: Expressing, Shear, Staining, Gene Expression, Quantitative RT-PCR, Two Tailed Test, Immunofluorescence, Western Blot

    Fig. 5. Piezo1 induces YAP activation through tyrosine 357 YAP and Src phosphorylation (A) and (B) Immunoblot analysis was measured after treatment with mock, a Piezo1 agonist (Yoda1, 1 μM), and an Src inhibitor (dasatinib, 100 nM) and exposure to static or WSS for 5 h. (C) and (D) Immunoblot analysis was measured after exposure to WSS for 5 h in PC3 cells transfected with siControl or siPiezo1. (B) and (D) Graphs showing quantification of the expression level of each phosphorylated protein compared with the expression of the non-phosphorylated isoform. (n = 3, Two Way ANOVA with Holm-Sidak multiple comparisons, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

    Journal: Life sciences

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.

    doi: 10.1016/j.lfs.2022.120936

    Figure Lengend Snippet: Fig. 5. Piezo1 induces YAP activation through tyrosine 357 YAP and Src phosphorylation (A) and (B) Immunoblot analysis was measured after treatment with mock, a Piezo1 agonist (Yoda1, 1 μM), and an Src inhibitor (dasatinib, 100 nM) and exposure to static or WSS for 5 h. (C) and (D) Immunoblot analysis was measured after exposure to WSS for 5 h in PC3 cells transfected with siControl or siPiezo1. (B) and (D) Graphs showing quantification of the expression level of each phosphorylated protein compared with the expression of the non-phosphorylated isoform. (n = 3, Two Way ANOVA with Holm-Sidak multiple comparisons, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

    Techniques: Activation Assay, Phospho-proteomics, Western Blot, Transfection, Expressing

    Fig. 6. In vivo monitoring of orthotopic PC3 pros tate tumor growth and metastasis with or without shPiezo1. (A) Representative whole-body images of bioluminescence measurements for xenografted PC3 tumor growth and metastasis. Luciferase tag ged PC3-Luc/shCon and PC3-Luc/shPiezo1 cells were surgically implanted into the dorsal prostate of male nude mice. Ventral images of anesthetized representative sham, control (PC3-Luc/shCon), and shPiezo1 (PC3-Luc/shPiezo1) were taken weekly for 6 weeks to monitor spreading to other organs. All images were pseudo color set using the same intensity (sham = 4, shCon = 6, shPiezo1 = 6). (B) Histological slides include orthotopic tumors of PC3-luc/shCon and PC3-luc/shPiezo1. Representa tive images were derived from mouse prostate stained with hematoxylin and eosin (H&E). High magnified images of the top panel were presented as below. Scale bars = 25 μm and 100 μm, respectively.

    Journal: Life sciences

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis.

    doi: 10.1016/j.lfs.2022.120936

    Figure Lengend Snippet: Fig. 6. In vivo monitoring of orthotopic PC3 pros tate tumor growth and metastasis with or without shPiezo1. (A) Representative whole-body images of bioluminescence measurements for xenografted PC3 tumor growth and metastasis. Luciferase tag ged PC3-Luc/shCon and PC3-Luc/shPiezo1 cells were surgically implanted into the dorsal prostate of male nude mice. Ventral images of anesthetized representative sham, control (PC3-Luc/shCon), and shPiezo1 (PC3-Luc/shPiezo1) were taken weekly for 6 weeks to monitor spreading to other organs. All images were pseudo color set using the same intensity (sham = 4, shCon = 6, shPiezo1 = 6). (B) Histological slides include orthotopic tumors of PC3-luc/shCon and PC3-luc/shPiezo1. Representa tive images were derived from mouse prostate stained with hematoxylin and eosin (H&E). High magnified images of the top panel were presented as below. Scale bars = 25 μm and 100 μm, respectively.

    Article Snippet: PC3, PC3-Luciferase (PC3-Luc2), and DU145 human prostate cancer cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

    Techniques: In Vivo, Luciferase, Control, Derivative Assay, Staining